Biotechnology in Agriculture

IMPORTANT

Biotechnology in Agriculture: Overview

This Topic covers sub-topics such as Recombinant DNA Technology, Tobacco Mosaic Virus, Transgenic Plants, Ti Plasmid, Genetically Modified Organisms, Marker Genes, Gene Gun, Biotechnological Applications in Agriculture and, Open Reading Frame

Important Questions on Biotechnology in Agriculture

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What are four methods of gene transfer?

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What is recombinant DNA technology ?

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Is Ti plasmid a cloning vector?

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_____ (Biolistic/Electroporation) is another name of gene gun.

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Electroporation is used as a physical method of gene transfer,

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Polyethylene glycol is used for the chemical method of gene transfer.

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_____ (Polyethylene glycol/Ascorbic acid) is used to transfer of gene in the plant.

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Tobacco mosaic virus is used in animal biotechnology.

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Explain the role of Tobacco Mosaic virus in plant biotechnology.

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Suggest how pathway engineering used to avoid the action of trehalose-6-phosphate in the production of citric acid.

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Amflora potatoes have high amount of protein molecules.

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State one advantage of Amflora potato.

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Write a note on open reading frame.

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What is the start codon of the open reading frame in RNA?

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Agrobacterium tumefaciens does not have any plasmid.

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 Write an importance of a marker gene.

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Fill in the blank with the correct answer from the bracket.

_____ helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants in genetic engineering. (Ori gene, marker gene, pBR322)

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Write the full form of the term GMO.

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'Bt' in the Bt gene that is used in producing insect-resistant plants refers to 'Biotechnology'.